Lab notebooks will be handed in through LON-CAPA. What is to be included in each lab report will be posted on the website under the syllabus link a week before lab reports are due. The TA's will go over this in detail the second week of lab.
The schedule of lab notebook due dates is included in the course syllabus and on the course website. Lab notebook assignments are due in LON-CAPA at 11:59pm on their due date.You are not allowed to work on a lab notebook assignment during the TA presentation given at the beginning of lab. Any reports turned in late will be scored as follows
a.)All late lab notebooks are to be turned into Renee Alt in 232 Burrill Hall by the day and time listed above. Do not turn in late notebooks to your TA.
- All MCB 301 students are required to keep a laboratory notebook. A notebook with duplicate numbered pages is required (see required materials for MCB 301). Full lab notebook assignments will typically be collected one week after completion of the exercise or per your teaching assistant’s instructions.
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Miniature circuit breaker construction is very simple, robust and maintenance-free. Generally, an MCB is not repaired or maintained, it just replaced by a new one when required. A miniature circuit breaker has normally three main constructional parts. These are: Frame of Miniature Circuit Breaker. The frame of a miniature circuit breaker is a.
The schedule of lab notebook due dates is included in the course syllabus and on the course website. Lab notebook assignments are due in LON-CAPA at 11:59pm on their due date.You are not allowed to work on a lab notebook assignment during the TA presentation given at the beginning of lab. Any reports turned in late will be scored as follows
1 minute to 48 hours late = 1/2 credit
Over 48 hours late = 0
Over 48 hours late = 0
Due Dates for Late Lab Notebooks scored for 1/2 credit
| Due on Monday | Due by 5:00pm on Wednesday |
Due on Tuesday | Due by 5:00pm on Thursday |
Due on Wednesday | Due by 5:00pm on Friday |
Due on Thursday | Scanned copy emailed to Renee Alt by 5:00pm on Saturday, paper copy due by 5:00pm on Monday |
a.)All late lab notebooks are to be turned into Renee Alt in 232 Burrill Hall by the day and time listed above. Do not turn in late notebooks to your TA.
Scientific writing is difficult for everyone in the beginning. It simply is not the style of writing that you are accustomed to using. Flowing, complex sentences that carry a level of nuance and elegance are gone, replaced by a series of concise, direct statements. In a word, scientific writing is boorish, dry prose largely devoid of the refinement and sophistication of language and grammar that students have had ingrained into them by teachers for years. As such, this short section of the laboratory manual was written to provide some form of guidance to you in writing your lab reports.
The most important thing to keep in mind is the cardinal rule of scientific writing: It must be concise and direct. There are no bonus points for writing a novel. In fact, writing a novel will hurt your performance far more than could ever help. Write as much as is needed to address the task at hand and be done with it. The second most important thing is to keep your writing organized. The bulk of this guide gives an example of how to organize your lab reports and what to include in each section.
SIGNIFICANCE
In previous lab courses, you most likely had a “Purpose” section for a lab report. The standard “purpose” statement often seen in lab reports written for teaching lines is as follows: The purpose of this exercise was to [re-state the title of the exercise]. While such a statement proves the student is capable of reading, it fails to demonstrate any understanding of why the exercise was done. Thus, the “Significance” section is used in this laboratory course as it is significantly different. There are in essence three aspects to the significance of a lab exercise: the theoretical goal, the pragmatic goal, and the hypothesis.
The theoretical goal of a laboratory exercise is the very broad idea that is encapsulated by the background material written in the laboratory manual. For example: In lab exercise #2, the theoretical goal is to understand how various staining techniques can be used to rapidly characterize unknown bacteria.
Clipwrap for mac free download. The pragmatic goal is the direct goal of the laboratory exercise. In lab exercise #2, it would be to understand the proper use and interpretation of Gram, acid-fast, spore, and capsule stain.
The hypothesis is what you are testing and what you believe the result will be. Due to the nature of the exercise, there is not a hypothesis for that particular lab.
METHODS
This section describes what you did during a particular lab exercise. Most of the time, it is assumed that you followed the procedures as written in this laboratory manual. This means that you need only cite the relevant section of the laboratory manual. For example: Gram staining of sample B was conducted as described on pages XX-XX of the laboratory manual. If you deviate from the procedures described in the manual, you should describe what aspects you changed.
Results
This section contains the observations that you made during the lab. Typically, a table is the simplest way to accomplish conveying your data to the reader. Keep in mind, that the goal of this section is simple to convey your direct observations, not the interpretation of those observations. For example, from laboratory exercise #2 you might have a table as such:
Staining Technique | Sample | Observation |
Gram Stain | 1A | 3 organisms: Cocci, in diplococci, pink Bacilli, in chains, purple Cocci, clustered, purple |
Acid-Fast Stain | 2B | 2 organisms: Bacilli, singly, red Cocci, clustered, purple |
Spore Stain How to crack hotbird channels. | 3B | 3 organisms: Bacilli, singly, pink cell body with green spherical regions Cocci, in chains, pink Cocci, singly, green |
Capsule Stain | 4A | 1 organism: Cocci, clustered, surrounding halo |
Discussion
This section contains your interpretation of the data that you reported in the “Results” section. Start with simple, concise statements. For example: Gram staining of sample 1A showed 3 organisms: a Gram negative diplococcus, a Gram positive streptobacillus, and a Gram positive staphylococcus.
In addition to interpreting your data, discuss data which do not make sense or which contradict your hypothesis. For example: If sample 1A contained a Gram negative diplococcus, it would like be a Neisseria species or Moraxella catarrhalis. All of which are too pathogenic for use in this teaching lab. It is more likely that the “Gram negative cocci” resulted from over use of ethanol during the decolorizing step.
Chemistry Lab Manual
Finally, each lab reports will that 2 or 3 discussions questions that you will need to address. These questions are more focused on conceptualizing the background material. You may have to look up some material outside of class, but you should be able to fully address each question in 2-3 sentences. If you find yourself writing a long paragraph, you should stop and ask yourself: what is the question actually asking and am I directly answering. Hedging your bet, so to speak, will not win you many points. Be confident; be concise; be direct. The goal of the questions is to help reinforce concepts, not trick you. In the words of one of my advisors: “Be 100% confident that you’re nearly always right.”
MCB7300: Molecular & Cellular Biology Laboratory
The purpose of this course is to expose students to a variety of laboratory techniques used in the broad field of molecular and cellular biology by allowing the student to carry out these techniques in the laboratory.
Revised Syllabus 2015
Day 1 Introduction to the Wyatt lab module (read web links below):
Bitesize web link
Flowchart-Wyatt 2014
Lab 1 Genomic DNA Extraction
Lab 2 Ligation Protocol
Lab 2 TA Cloning
